| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1221 | Holdemania filiformis | TCAAGCTGCTGCGGTTGA | TCCCCAAACCGATGCTGAC | 59.89 | 60.00 | 258 | 40 |
100.00%
|
50.00%
|
| Primer 1222 | Holdemania filiformis | AGCATGGCAAGCTGGCTT | AAACTGCAGCGCATCCCA | 60.28 | 60.28 | 218 | 40 |
100.00%
|
50.00%
|
| Primer 1223 | Holdemania filiformis | ATCGCGTCGGAAGCTCAA | AACTGGCAGAGTCATCCGC | 59.43 | 60.08 | 255 | 40 |
100.00%
|
50.00%
|
| Primer 1224 | Holdemania filiformis | GCGCAAACACAGAACCGA | TTGCCCAACCCGACGATT | 58.98 | 59.57 | 294 | 40 |
100.00%
|
50.00%
|
| Primer 1225 | Holdemania filiformis | CGCAAAGCAACGAACCGA | TCCGTTTCCACACCGCAA | 59.37 | 59.81 | 253 | 40 |
100.00%
|
50.00%
|
| Primer 1226 | Holdemania filiformis | CGCAAAGCAACGAACCGA | TTCCGTTTCCACACCGCA | 59.37 | 59.81 | 254 | 40 |
100.00%
|
50.00%
|
| Primer 1227 | Holdemania filiformis | TTTCAACGACCGCACGGA | TTCCGCTTCCGCTTTCGA | 59.89 | 59.66 | 178 | 40 |
100.00%
|
50.00%
|
| Primer 1228 | Holdemania filiformis | TCCTGCAGGAGATCGTCGA | AAATGGACGGCTTGCGGA | 60.08 | 59.97 | 247 | 40 |
100.00%
|
50.00%
|
| Primer 1229 | Holdemania filiformis | GTGGCTGCTCGATCCCAAT | ACGCTTGAACGCCTCCTT | 60.15 | 59.57 | 155 | 40 |
100.00%
|
50.00%
|
| Primer 1230 | Holdemania filiformis | TGGCTGCTCGATCCCAAT | ACGCTTGAACGCCTCCTT | 58.68 | 59.57 | 154 | 40 |
100.00%
|
50.00%
|